MultiFlo™ FX is an automated reagent dispenser for 6- to 1536-well microplates. Its unique Parallel Dispense™ technology allows up to four independent reagents to be dispensed in parallel without potential carryover. What makes MultiFlo FX “multi-mode” is its configurability: you can add a wash module or the RAD™ module, for random access individual well dispensing and bulk dispensing larger wells. Everything you need for fast, intuitive programming and operation is available through the color touchscreen. MultiFlo FX configured with either the wash module or RAD technology replaces up to five liquid handlers in a single compact platform.
Multi-mode dispensing can replace up to four dispensers and a washer
Parallel Dispense™ technology: peristaltic and/or syringe pumps
RAD™ technology for dispensing to individual wells
Cost savings through low prime volume and single tube access
Cell friendly angled dispense and/or wash tubes won’t disrupt cell layers
Walkaway automation with BioStack 4, for lidded or unlidded plates
Microplate types
Dispensing (peri pump and syringe)
96-, 384- and 1536-well standard, deep and PCR plates; 6-. 12-, 24- and 48-well plates (dispense tip configurable)
Washing
96-. 384-well standard plates; 6-, 12-, 24-,and 48-well plates (with compatible manifold configuration)
Onboard software (computer control)
Create, edit or run multiple protocols
Software LHC2 Software
LHC2 Secure for 21 CFR Part 11 compliance (option)
SiLA Compliant driver (option)
Shaking Programmable up to 60 minutes
Slow, medium, fast or variable
Soaking Programmable up to 60 minutes
USB ports (2) For protocol storage/transfer and for optional external mouse or keyboard
Automation BioStack and 3rd party automation compatible
BioSpa 8 Automated Incubator compatible
Washing
Manifold types 96- and 384-well washing: 8-tube manifold
6-, 12-, 24-, 48-well washing: Custom manifolds available
Volume range 20-30,000 µL/well
Wash cycles 1-10
Supply bottle 2 L
Dispense accuracy +3%
Dispense precision 96-/384-well plates, 300 µL/well: <3% CV
6-well plates, 5560 µL/well: <5% CV
Residual volume 96-well plate, 300 µL/well: < 2 µL/well
Wash speed 96-wells, 8-tube manifold, 3 cycles, >300 µL/well: <130 seconds
Wash volume 20-30,000 µL/well
Waste bottle 2L
Flow rates 140-422 µL/well/second
Sterilization Chemical
Dispensing - Peristaltic pump (Multi-Channel)
Manifold types 1 x 8 - sapphire jeweled 316 SS, 316 SS or polypropylene tips, with 1, 5 or 10 µL tubing
Fluid delivery 1 or 2 peristaltic pumps
Dispense speed
96 wells, 5 µL cass, 10 µL/ well: 3 seconds
96 wells, 5 µL cass, 20 µL/ well: 3.5 seconds
384 wells, 5 µL cass, 5 µL/ well: 6.5 seconds
384 wells, 10 µL cass, 10 µL/ well: 8 seconds
384 wells, 1 µL cass, 1 µL/ well: 6 seconds
1536 wells, 1 µL cass, 1 µL/ well: 21 seconds
Volume range 500 nL - 3,000 µL/well, selectable in 1 µL increments
Flow rates User programmable rates from high to low
Dispense performance
1 µL cassette: recommended range: 1 - 50 µL
Accuracy: +5% at 1 µL
Precision: <5% CV at 1 µL
<10% CV at 500 nL
Minimum Prime Volume: 1.20 mL
5 µL cassette: recommended range: 5 - 2,500 µL
Accuracy: +2.0% at 5 µL
Precision: <2.5% CV at 5 µL
Minimum Prime Volume: 4.23 mL
10 µL cassette: recommended range: 10 - 3,000 µL
Dispense Accuracy: +2.0% at 10 µL
Dispense Precision: <2.0% CV at 10 µL
Minimum Prime Volume: 7.36 mL
Recommended cassette replacement interval 1 µL Cassette: 1,000 384-well microplates at 5 µL well
5 µL Cassette: 1,000 96-well microplates at 50 µL well
10 µL Cassette: 1,000 96-well microplates at 100 µL well
Sterilization Autoclave, chemical
Dispensing - Syringe pump
Manifold types
96- and 384-well dispensing:
One 16-tube (2 x 8) manifolds - 316 SS tubes
Two 16-tube (1 x 16) manifolds - 316 SS tubes
1536-well dispensing:
Two 32-tube (1 x 32) manifolds - sapphire jeweled 316 SS or 316 SS tubes
6- to 48-well dispensing: custom autoclavable manifolds available
Fluid delivery Two positive displacement syringe drives
Dispense speed
20 µL /well, 96 wells, 1 x16 tubes:: 5 seconds
20 µL /well, 384 wells, 1 x16 tubes:: 14 seconds
3 µL /well, 1536 wells, 2 x 32 tubes:: 7 seconds
Volume range 3 - 3,000 µL/well selectable in 1 µL increments
Minimum Prime Volume: 12 mL
Flow rates User programmable rates from high to low
Dispense accuracy +1 µL at 5 µL
+1 µL at 20 µL
+1% at 100 µL
Dispense precision <5% CV at 5 µL
<2.5% CV at 20 µL
<1% CV at 100 µL
Supply bottle 1 L or 2 L
Sterilization Chemical, autoclavable option
Dispensing - Peristaltic pump (RAD Technology)
Labware types
Single tip: 6-, 12-, 24-, 48-, 96-, 384-well plates; low profile
standard height and deep well formats; PCR trays and microtubes
8-to-1 tip: 6-, 12-, and 24-well plates
Manifold Types
RAD single, with plastic or steel tip with 1, 5 or 10 tubing, 7° angle
RAD 8-to-1 plastic tip, with 5 µL tubing, angled bulk dispense chute
Volume range 500 nL - 30,000 µL
Minimum prime volume
1 µL cass, 18": 90 µL ; 1 µL cass, 30": 150 µL
5 µL cass, 18": 320 µL; 1 µL cass, 30": 530 µL
10 µL cass, 18": 555 µL; 10 µL cass, 30": 920 µL
Dispense speed (high flow)
1 µL cass, 1 µL/well: 19s (96 wells) 55s (384 wells)
1 µL cass, 10 µL/well: 33s (96 wells), 112s (384 wells)
5 µL cass, 5 µL/well: 19s (96 wells), 58s (384 wells)
5 µL cass, 100 µL/well: 76s (96 wells), 286s (384 wells)
10 µL cass, 10 µL/well: 21s (96 wells), 66s (384 wells)
10 µL cass, 100 µL/well: 70s (96 wells), 259s (384 wells)
Dispense performance
1 µL cass (med), 0.5 µL/well: Precision 10% CV
1 µL cass (med), 1 µL/well: Accuracy + 10%, Precision 10% CV
1 µL cass (med), >2 µL/well: Accuracy + 5%, Precision 5% CV
5 µL cass (high), 5 µL/well: Accuracy + 4%, Precision 5% CV
5 µL cass (high), >10 µL/well: Accuracy + 2%, Precision 2.5% CV
10 µL cass (high), 10 µL/well: Accuracy + 4%,Precision 4% CV
10 µL cass (high), >20 µL/well: Accuracy + 2%, Precision 2 %CV
8-to 1 cass(high), 40 µL/well: Accuracy + 4%
8-to 1 cass (high), >10 µL/well: Precision 2.5%CV
8- to 1cass(high), >80 µL/well: Accuracy + 2%
Physical Characteristics
Power 100 - 240 Volts AC. 50/60 Hz
Dimensions Base instrument: 17.19"W x 11.75" D x 8" H (43.51 x 29.21 x 20.32 cm)
Weight Base instrument:
19.5 lbs (8.8 Kg)
Regulatory CE and TUV marked, RoHS Compliant. Models for In Vitro Diagnostic use are available.
