The NEPHELOstar Plus is a microplate nephelometer with a high-intensity laser diode. It can be up to thirty times more sensitive than traditional transmission optical density readers that measure the reduction in direct light passing through a sample well. Thanks to its speed, flexibility, and performance, the NEPHELOstar Plus allows more applications to be adapted to microplate-based laser nephelometry than ever before.
Features
- Adjustable laser intensity and beam diameter
- Measurement in up to 384-well microplate format
- Incubation up to 45°C or 60°C
- Linear, orbital and double-orbital shaking
- High-precision injectors with simultaneous reagent injection and detection
- Microplate Stacker and robot compatible
- Easily integrated into automated systems
Detection Modes
Nephelometry (light scattering)
Measurement Modes
Endpoint and kinetic
Microplate Formats
Up to 384-well plates
Microplate Carrier
Robot compatible
Light Sources
Self-monitoring laser diode
Wavelength 635±10 nm
Stability <0.2% deviation
Lifetime 20,000 hours
Output: 1 mW
Selectable beam width: 1.5 to 3.5 mm
Selectable intensity 0-100%
Scattering angle: detects up to 80° full cone angle
Detectors
Side window photodiode detector
Wavelength Selection
Photodiode
Wavelength 635±10 nm
Sensitivity
Depending on particle size and liquid properties
Silica detection (particle size 0.5 to 10 µm) 800 nM
Dynamic Range: 5 decades
Maximum count rate (2,000,000 Relative Nephelometry Units (RNUs) per second)
Read Times
Depend on assay conditions and liquid surface stability
Shortest times possible: 16 s (96), 47 s (384)
Shaking
Linear, orbital, and double-orbital with user-definable time and speed
Purge Gas Vent
System to inject an atmosphere or to pull a vacuum into the reader
Incubation
+4 °C above ambient up to 45 °C or 60 °C
The upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer
Software
Multi-user Reader Control and MARS Data Analysis Software included
FDA 21 CFR Part 11 compliant
Dimensions
Width: 44 cm, depth: 48 cm, height: 32 cm; weight: 25 kg
Reagent Injection
Up to 2 built-in reagent injectors Injection at measurement position (6 to 384-well) Individual injection volumes for each well (3 to 500 µL) Variable injection speed up to 420 µL/s Up to four injection events per well
Reagent back flushing
