Description Specification
Block configurations and volume • 96-well (10–100 μL reactions),
• Fast 96-well (15–30 μL reactions)
• 384-well (5–20 μL reactions)
• TaqMan® Array Cards (~1 μL reactions)
• OpenArray® Plates (33 nL reactions)
Block change design Block change from front in less than 1 min; no tools or service call required
Excitation source Enhanced OptiFlex® system, White LED
Instrument control Instrument touch screen, networked computer, or attached computer
Detection channels • Decoupled: 6 emission, 6 excitation (96-well/Fast, 384-well, TaqMan® blocks)
• Coupled: 4 emission, 4 excitation (OpenArray® blocks)
21 CFR p11 enablement Optional software module
Dimensions (W x D x H) 50.5 cm x 67.2 cm x 73.8 cm
Weight 69 kg (152 lbs)
Power 100–240 V
Remote monitoring Available to monitor up to 15 networked instruments simultaneously
Data export format User configurable: *.xls, *xlsx, *.txt
96-well, 96-well Fast, 384-well,
TaqMan® Array Card blocks OpenArray® block
Detection channels Decoupled: 6 emission, 6 excitation Coupled: 4 emission, 4 excitation
Well-to-well variability +/– 0.25°C +/– 0.75°C
Max block ramp rate 3.0°C/sec (384-well) 3.0°C/sec
Run time • 30 min expected (Fast 96-well block)
• 35 min (384-well block, using Fast
master mix)
• 2 hr (gene expression)
• 4 hr (genotyping)
Demonstrated sensitivity To 1 copy To 1 copy
Dynamic range To 9 logs To 7 logs
Resolution As low as 1.5-fold change for single-plex
reaction
As low as 2-fold change for single-plex
reaction
