The Applied Biosystems QuantStudio 5 Real-Time PCR System for Human Identification with tower computer is the latest addition to our human identification (HID) real-time PCR family of products. This instrument is designed for both new and experienced users who need a simple, reliable, and affordable real-time PCR system that doesn’t require compromise on performance or quality. When used with our latest advances in quantification chemistry and HID Real-Time PCR Analysis Software, this system offers a sensitive, robust solution for forensic DNA quantification. It also helps prepare laboratories for future quantification technologies. With maximum dye versatility, this HID solution offers accurate, trusted results in a small benchtop footprint.
Features of the QuantStudio 5 Real-Time PCR System for Human Identification:
Streamlined
• Smaller foot print: 27 W x 50 D x 40 H cm, designed to maximize horizontal and vertical bench space
• Front-facing fully motorized block
Interactive
• Touch screen interface enables viewing of amplification plots
• Easy and intuitive interface
Reliable
• Bright light LED median lifetime greater than 5 years
• Less frequent maintenance
• 10 GB on-board memory for storage of 2,000–5,000 runs
• HID-validated system
• 0.2 mL standard 96-well format
Innovative
• Multiplexing capability for up to six dyes, enabling future quantification technology
• Precise temperature control with Peltier-controlled blocks (known as VeriFlex zones)
• USB interface
Real-Time PCR applications
Real-time PCR is a flexible technique that can be used for multiple forensic applications:
• Forensic database
--Laboratories without direct amplification workflows
• Forensic casework
--Confirmatory screening of male DNA
--Sexual assault sample workflow
--Quality and quantity sample assessment
Specifications
Shipping Condition
Room Temperature
Block Format
Non-Interchangeable
Display Type
Touch Screen
Excitation (light source) Bright white light-emitting diode (LED) source with a median
lifetime of >5 years
Dimensions and weight 27 x 50 x 40 cm (W x D x H), <26 kg
Sample capacity (wells) 96-well 0.2 mL block
Reaction volume 96-well: 10–100 μL for 0.2 mL block
Maximum ramp rate 6.5°C/sec
Average sample ramp rate 3.66°C/sec
Temperature uniformity 0.4°C
VeriFlex™ Blocks 96-well: 6 independent temperature zones
Heating/cooling method Peltier
Run time ~70 min for Applied Biosystems™ Quantifiler™ Trio and HP kits
~100 min for Applied Biosystems™ Quantifiler™ Duo and
Human kits
Calibration Factory-calibrated*
Onboard memory 10 GB, which translates to approximately 2,000–5,000 run files
Electrical approvals IEC, CE
