The AutoDG Instrument partitions each sample in a 96-well PCR plate into ~20,000 uniform nanoliter-sized droplets in which nucleic acid molecules are randomly distributed. Every droplet serves as an individual PCR reaction in Bio-Rad’s Droplet Digital (ddPCR) PCR System. After partitioning, the plate is sealed with the PX1 Plate Sealer, and PCR is run to end point in a thermal cycler. The droplets in each sample can then be analyzed using a QX600 or QX200 Droplet Reader.
- Eliminates user-to-user variability — creates more consistent droplet counts across users and plates, enabling you to fully exploit the precision of your ddPCR System
- Minimizes hands-on time — takes less than 5 minutes to load the instrument, and droplet generation requires no supervision
Features and Benefits of the Automated Droplet Generator
Bio-Rad’s Automated Droplet Generator simplifies ddPCR workflow and makes digital PCR scalable and flexible:
- Guides setup and loading with large, color touch-screen interface and LED lighting
- Generates droplets for 96 ddPCR reactions in under 45 minutes
- One AutoDG Instrument can generate sufficient droplets to keep 4–5 droplet readers running continuously
- Provides operational flexibility:
- User-definable plate layout with increments of 8 wells
- HEPA-filtered enclosure reduces the possibility of contamination and allows the system to be placed in any lab environment
- Compatible with both QX600 and QX200 ddPCR workflows
Power:100–240 V, 50–60 Hz, 221W
Weight: 100 lb (45.4 kg)
Dimensions: 26 in W x 22 in D x 26 in H (66 cm x 56 cm x 66 cm)
Usage temperature: 18–30° C, optimal range is 23 ± 2° C
Relative humidity: 50% max
Sample capacity: 1–96 samples per run
Droplets per 20 µl sample: 20,000
Sample illumination: Light-emitting diodes
Sample detection: Multi-pixel photon counter
Precision: ±10%
Linear dynamic range: 5 orders of magnitude
