The QX200 AutoDG Droplet Digital PCR System provides absolute quantification of target DNA or RNA molecules for EvaGreen and probe-based Droplet Digital PCR (ddPCR) applications. The Automated Droplet Generator simplifies the ddPCR workflow, making digital PCR both scalable and practical.
The AutoDG Instrument eliminates user-to-user variability, allowing you to fully exploit the precision of ddPCR technology by providing more consistent droplet counts across users and plates. The AutoDG Instrument also minimizes hands-on time — it takes less than 5 minutes to load the instrument, and no supervision or user activity is required during droplet generation.
Features and Benefits of the QX200 AutoDG Droplet Digital PCR System
Automated Droplet Generator — simplifies the ddPCR workflow and offers flexibility:
- Generates droplets for 96 ddPCR reactions in under 45 minutes
- Compatible with both the QX100 and QX200 Droplet Digital PCR Systems
- A single AutoDG Instrument can supply 4–5 Droplet Readers continuously
- Guides setup and loading with large, color touch-screen interface and LED lighting
- User-definable plate layout with increments of 8 wells provides run-to-run flexibility
- HEPA-filtered enclosure reduces the possibility of contamination and allows the system to be placed in any lab environment
Droplet Digital PCR Technology — the most precise and sensitive digital PCR solution for a wide variety of applications, providing:
- Flexible digital PCR chemistry — optimized for TaqMan Hydrolysis Probe and EvaGreen Assays
- Nanoliter droplet partitioning — reduces bias from amplification efficiency differences and PCR inhibitors
- Convenient assay design — standard curves are not required
Specifications
Starting sample size, µl
20
QX200 Droplet Generator capacity
1–8 samples/cartridge
Droplets per 20 µl sample
20,000
QX200 droplet reader capacity
1–96 samples
Sample illumination
Light-emitting diodes
Sample detection
Multi-pixel photon counter
Detection channels
FAM (EvaGreen), HEX (VIC)
Linear dynamic range
5 orders of magnitude
Precision
±10%
Droplets per 96-well plate, million
~1.5
QX200 droplet generator dimensions (W x D x H)
28 x 36 x 13 cm (11 x 14 x 5")
QX200 droplet reader dimensions (W x D x H)
66 x 52 x 29 cm (26 x 20 x 11")
